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The t-Boc amino acid derivatives had been used dominantly for the solid phase peptide
synthesis (SPPS) until the
Fmoc chemistry was introduced to overcome the t-Boc chemistry problems, repetitive TFA treatment which might catalyze the
side reactions and cleave the peptide chain prematurely. In Fmoc synthesis, the growing peptide is subjected to mild base
treatment using piperidine to remove the Fmoc group, and TFA is required only for the final deprotection and cleavage from
the resin. The comparison experiments between Fmoc and t-Boc
approach indicate that Fmoc chemistry is more reliable
and tends to give the desired peptides in better purities. |
